| 貨號 |
名稱 |
同裂酶 |
異裂酶 |
識別位點 |
切割末端 |
反應溫度 |
失活條件 |
甲基化影響 |
|
GMP501S
|
BsaI, GMP Grade |
Eco31I, Bso31I, BspTNI |
|
GGTCTC(1/5)
5'...G G T C T C (N)? ↓...3'
3'...C C A G A G (N)? ↑...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: impaired.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
|
|
GMP503S
|
BspQI, GMP Grade |
SapI, LguI, PciSI |
|
GCTCTTC(1/4)
5'...G C T C T T C (N)?↓...3'
3'...C G A G A A G (N)?↑...5' |
5’ protruding ends |
50℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
GMP505S
|
NheI, GMP Grade |
AsuNHI |
BmtI, BspOI |
GCTAGC
5'...G ↓ C T A G C...3'
3'...C G A T C ↑ G...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: some blocked.
EcoKI: no effect.
EcoBI: no effect.
|
|
GMP504S
|
XhoI, GMP Grade |
PaeR7I, Sfr274I, SlaI |
|
CTCGAG
5'...C ↓ T C G A G...3'
3'...G A G C T ↑ C...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
|
|
GMP502S
|
XbaI, GMP Grade |
|
|
TCTAGA
5'...T ↓ C T A G A...3'
3'...A G A T C ↑ T...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: blocked.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG25511S
|
LightNing® AatII |
|
ZraI |
GACGTC
5'...G A C G T ↓ C...3'
3'...C ↑ T G C A G...5' |
3’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15502S
|
LightNing® AccI |
XmiI, FblI |
|
GTMKAC
5'...G T ↓ M K A C...3'
3'...C A K M ↑ T G...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG25501V
|
LightNing® AciI |
BspACI, SsiI |
|
CCGC
5'...C ↓ C G C...3'
3'...G G C ↑ G...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG24520S
|
LightNing® AflII |
BspTI, BfrI, BstAFI, MspCI, Vha464I |
|
CTTAAG
5'...C ↓ T T A A G...3'
3'...G A A T T ↑ C...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG23501S
|
LightNing® AgeI |
AsiGI, CspAI, BshTI, PinAI |
|
ACCGGT
5'...A ↓ C C G G T...3'
3'...T G G C C ↑ A...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG24509S
|
LightNing® AluI |
AluBI |
|
AGCT
5'...A G ↓ C T...3'
3'...T C ↑ G A...5' |
blunt |
37℃ |
80℃，20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: blocked.
|
|
EG25502S
|
LightNing® ApaI |
Bsp120I, PspOMI |
|
GGGCCC
5'...G G G C C ↓ C...3'
3'...C ↑ C C G G G...5' |
3’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: some blocked.
CpG: some blocked.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15601S
|
LightNing® ApaLI |
Alw44I, VneI |
|
GTGCAC
5'...G ↓ T G C A C...3'
3'...C A C G T ↑ G...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: impaired.
EcoBI: no effect.
|
|
EG15508S
|
LightNing® AscI |
PalAI, SgsI |
|
GGCGCGCC
5'...G G ↓ C G C G C C...3'
3'...C C G C G C ↑ G G...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG25503S
|
LightNing® AseI |
PshBI, VspI |
|
ATTAAT
5'...A T ↓ T A A T...3'
3'...T A A T ↑ T A...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: impaired.
|
|
EG25509S
|
LightNing® AvaII |
Bme18I, Eco47I |
|
GGWCC
5'...G ↓ G W C C...3'
3'...C C W G ↑ G...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: blocked.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15509S
|
LightNing® AvrII |
AspA2I, BlnI, XmaJI |
|
CCTAGG
5'...C ↓ C T A G G...3'
3'...G G A T C ↑ C...5' |
5’ protruding ends |
37℃ |
No (column purification) |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15510S
|
LightNing® BamHI |
|
|
GGATCC
5'...G ↓ G A T C C...3'
3'...C C T A G ↑ G...5' |
5’ protruding ends |
37℃ |
No (column purification) |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG24512S
|
LightNing® BbsI |
BstV2I, BpiI |
|
GAAGAC(2/6)
5'...G A A G A C (N)?↓...3'
3'...C T T C T G (N)?↑...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15513S
|
LightNing® BclI |
FbaI, Ksp22I |
|
TGATCA
5'...T ↓ G A T C A...3'
3'...A C T A G ↑ T...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: blocked.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: some blocked.
|
|
EG24507S
|
LightNing® BglI |
|
|
GCCNNNNNGGC
5'...G C C N N N N ↓ N G G C...3'
3'...C G G N ↑ N N N N C C G...5' |
3’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15591S
|
LightNing® BglII |
|
|
AGATCT
5'...A ↓ G A T C T...3'
3'...T C T A G ↑ A...5'
|
5’ protruding ends |
37℃ |
No (column purification) |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: some blocked.
|
|
EG15518S
|
LightNing® BsaI |
Eco31I, Bso31I, BspTNI |
|
GGTCTC(1/5)
5'...G G T C T C (N)? ↓...3'
3'...C C A G A G (N)? ↑...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: impaired.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG25507S
|
LightNing® BsmI |
BsaMI, Mva1269I, PctI |
|
GAATGCN
5'...G A A T G C N ↓...3'
3'...C T T A C↑G N ...5' |
3’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: blocked.
|
|
EG25512S
|
LightNing® BspHI |
CciI, PagI |
|
TCATGA
5'...T ↓ C A T G A...3'
3'...A G T A C ↑ T...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: impaired.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: impaired.
|
|
EG23505S
|
LightNing® BsrGI |
BstAUI, Bsp1407I |
|
TGTACA
5'...T ↓ G T A C A...3'
3'...A C A T G ↑ T...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15607S
|
LightNing® BstBI |
AsuII, Bpu14I, Bsp119I, BspT104I, NspV, SfuI |
|
TTCGAA
5'...T T ↓ C G A A...3'
3'...A A G C ↑ T T...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: some blocked.
EcoBI: no effect.
|
|
EG15528S
|
LightNing® BstEII |
BstPI，Eco91I，EcoO65I，PspEI |
|
GGTNACC
5'...G ↓ G T N A C C...3'
3'...C C A N T G ↑ G...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: impaired.
EcoBI: impaired.
|
|
EG15532S
|
LightNing® ClaI |
BspDI，Bsa29I，BseCI，BshVI，Bsu15I，BsuTUI |
|
ATCGAT
5'...A T ↓ C G A T...3'
3'...T A G C ↑ T A...5'
|
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: blocked.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: impaired.
|
|
EG25508S
|
LightNing® Cfr42I (SacII) |
SacII，KspI，Sfr303I，SgrBI |
|
CCGCGG
5'...C C G C ↓ G G...3'
3'...G G ↑ C G C C...5' |
3’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG24501S
|
LightNing® DdeI |
BstDEI, HpyF3I |
|
CTNAG
5'...C ↓ T N A G...3'
3'...G A N T ↑ C...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: blocked.
|
|
EG15585S
|
LightNing® DpnI |
MalI |
Bsp143I, BssMI, BstKTI, BstMBI, DpnII, Kzo9I, MboI, NdeII, Sau3AI |
GA??TC
5'...G A?? ↓ T C...3'
3'...C T ↑ A?? G...5' |
blunt |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: impaired.
|
|
EG15533S
|
LightNing® DpnII |
MboI，Sau3AI，Bsp143I, BssMI，BstMBI，Kzo9I，NdeII |
BstKTI, DpnI, MalI |
GATC
5'...↓G A T C...3'
3' ... C T A G↑...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: blocked.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: blocked.
|
|
EG23502S
|
LightNing® DraI |
|
|
TTTAAA
5'...T T T ↓ A A A...3'
3'...A A A ↑ T T T ..5' |
blunt |
37℃ |
80℃，20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: blocked.
EcoBI: no effect.
|
|
EG24508S
|
LightNing® DraIII |
AdeI |
|
CACNNNGTG
5'...C A C N N N ↓ G T G...3'
3'...G T G ↑ N N N C A C...5' |
3’ protruding ends |
37℃ |
No (column purification) |
Dam: no effect.
Dcm: no effect.
CpG: some impaired.
EcoKI: impaired.
EcoBI: some blocked.
|
|
EG15535S
|
LightNing® EagI |
BseX3I, BstZI, EclXI, Eco52I |
|
CGGCCG
5'...C ↓ G G C C G...3'
3'...G C C G G ↑ C ..5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG23511S
|
LightNing® EarI |
Eam1104I, Bst6I |
|
CTCTTC(1/4)
5'...C T C T T C (N)?↓...3'
3'...G A G A A G (N)?↑...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15536S
|
LightNing® EcoRI |
FunII |
|
GAATTC
5'...G ↓ A A T T C...3'
3'...C T T A A ↑ G...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: impaired.
|
|
EG15537S
|
LightNing® EcoRV |
Eco32I |
|
GATATC
5'...G A T ↓ A T C...3'
3'...C T A ↑ T A G...5' |
blunt |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: some impaired.
EcoKI: no effect.
EcoBI: some blocked.
|
|
EG21503S
|
LightNing® Esp3I (BsmBI) |
BsmBI |
|
CGTCTC(1/5)
5'...C G T C T C (N)?↓...3'
3'...G C A G A G (N)?↑...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG24523S
|
LightNing® FokI |
|
BtsCI, BstF5I, BseGI |
GGATG(9/13)
5'...G G A T G (N)? ↓...3'
3'...C C T A C (N)??↑...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: some impaired.
CpG: some impaired.
EcoKI: no effect.
EcoBI: impaired.
|
|
EG15586S
|
LightNing® FspI |
Acc16I,NsbI |
|
TGCGCA
5'...T G C ↓ G C A...3'
3'...A C G ↑ C G T...5' |
blunt |
37℃ |
No (column purification) |
Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG23509S
|
LightNing® HaeIII |
BsuRI, BshFI, BspANI, BsnI |
AoxI |
GGCC
5'...G G ↓ C C...3'
3'...C C ↑ G G...5' |
blunt |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG24510S
|
LightNing® HhaI |
HinP1I, CfoI, AspLEI, Hin6I, HspAI, BstHHI |
|
GCGC
5'...G C G ↓ C...3'
3'...C ↑ G C G...5' |
3’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15539S
|
LightNing® HindIII |
|
|
AAGCTT
5'...A ↓ A G C T T...3'
3'...T T C G A ↑ A...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: some blocked.
|
|
EG15593S
|
LightNing® HinfI |
|
|
GANTC
5'...G ↓ A N T C...3'
3'...C T N A ↑ G...5'
|
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: blocked.
|
|
EG24511S
|
LightNing® HinP1I |
HhaI, CfoI, AspLEI, Hin6I, HspAI, BstHHI |
|
GCGC
5'...G ↓ C G C...3'
3'...C G C ↑ G...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15541S
|
LightNing® HpaI |
KspAI |
|
GTTAAC
5'...G T T ↓ A A C...3'
3'...C A A ↑ T T G...5' |
blunt |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: impaired.
EcoBI: no effect.
|
|
EG23507S
|
LightNing® HpaII |
MspI, HapII, BsiSI |
|
CCGG
5'...C ↓ C G G...3'
3'...G G C ↑ C...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15543S
|
LightNing® KasI |
SspDI |
Mly113I, EgeI, NarI, EheI, DinI, SfoI, PluTI |
GGCGCC
5'...G ↓ G C G C C...3'
3'...C C G C G ↑ G...5' |
blunt |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15544S
|
LightNing® KpnI |
|
Asp718I, Acc65I |
GGTACC
5'...G G T A C ↓ C...3'
3'...C ↑ C A T G G...5' |
3’ protruding ends |
37℃ |
No (column purification) |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG24522S
|
LightNing® MboI |
Bsp143I, Kzo9I, BssMI, DpnII, BstMBI, NdeII, Sau3AI |
BstKTI |
GATC
5'...↓G A T C ...3'
3'... C T A G↑...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: blocked.
Dcm: no effect.
CpG: some impaired.
EcoKI: no effect.
EcoBI: some blocked.
|
|
EG15547S
|
LightNing® MluI |
|
|
ACGCGT
5'...A ↓ C G C G T...3'
3'...T G C G C ↑ A...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: impaired.
EcoBI: impaired.
|
|
EG15548S
|
LightNing® MnlI |
|
|
CCTC(7/6)
5'...C C T C N? ↓...3'
3'...G G A G N? ↑...5' |
3’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: blocked.
|
|
EG25505S
|
LightNing® MscI |
BalI, MlsI, Mox20I, Msp20I |
|
TGGCCA
5'...T G G ↓ C C A...3'
3'...A C C ↑ G G T...5' |
blunt |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: blocked.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG24518S
|
LightNing® MseI |
SaqAI, Tru9I, Tru1I |
|
TTAA
5'...T ↓ T A A...3'
3'...A A T ↑ T...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: some blocked.
EcoBI: no effect.
|
|
EG23508S
|
LightNing® MspI |
HpaII, HapII, BsiSI |
|
CCGG
5'...C ↓ C G G...3'
3'...G G C ↑ C...5' |
5’ protruding ends |
37℃ |
No (column purification) |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG23510S
|
LightNing® MunI (MfeI) |
MfeI |
|
CAATTG
5'...C ↓ A A T T G...3'
3'...G T T A A ↑ C...5' |
5’ protruding ends |
37℃ |
No (column purification) |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15550S
|
LightNing® NcoI |
Bsp19I |
|
CCATGG
5'...C ↓ C A T G G...3'
3'...G G T A C ↑ C...5'
|
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15551S
|
LightNing® NdeI |
FauNDI |
|
CATATG
5'...C A ↓ T A T G...3'
3'...G T A T A ↑ C...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15552S
|
LightNing® NheI |
AsuNHI |
BmtI, BspOI |
GCTAGC
5'...G ↓ C T A G C...3'
3'...C G A T C ↑ G...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: some blocked.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15553S
|
LightNing® NotI |
CciNI |
|
GCGGCCGC
5'...G C ↓ G G C C G C...3'
3'...C G C C G G ↑ C G...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15602S
|
LightNing® NruI |
Bsp68I，BtuMI，RruI |
|
TCGCGA
5'...T C G ↓ C G A...3'
3'...A G C ↑ G C T...5' |
blunt |
37℃ |
No (column purification) |
Dam: blocked.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15606S
|
LightNing® NsiI |
EcoT22I, Mph1103I, Zsp2I |
|
ATGCAT
5'...A T G C A ↓ T...3'
3'...T ↑ A C G T A...5' |
3’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: some impaired.
|
|
EG15603S
|
LightNing® PacI |
|
|
TTAATTAA
5'...T T A A T ↓ T A A...3'
3'...A A T ↑ T A A T T...5'
|
3’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG23506S
|
LightNing® PmeI |
MssI |
|
GTTTAAAC
5'...G T T T ↓ A A A C...3'
3'...C A A A ↑ T T T G...5' |
blunt |
37℃ |
80℃,20 min |
Dam: no effect.
Dcm: no effect.
CpG: some blocked.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG25510S
|
LightNing® PmlI |
AcvI, Eco72I, PspCI, BbrPI |
|
CACGTG
5'...CAC ↓ GTG...3'
3'...GTG ↑ CAC ..5' |
blunt |
37℃ |
80℃，20 min |
Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: some blocked.
EcoBI: some blocked.
|
|
EG15560S
|
LightNing® PspGI (EcoRII) |
EcoRII, Psp6I, AjnI |
BciT130I, BseBI, Bst2UI, BstNI, MvaI |
CCWGG
5'...↓C C W G G ...3'
3'... G G W C C↑...5' |
5’ protruding ends |
75℃ |
No (column purification) |
Dam: no effect.
Dcm: blocked.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15561S
|
LightNing® PstI |
BspMAI |
|
CTGCAG
5'...C T G C A ↓ G...3'
3'...G ↑ A C G T C...5' |
3’ protruding ends |
37℃ |
No (column purification) |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG23513S
|
LightNing® PvuI |
Ple19I |
|
CGATCG
5'...C G A T ↓ C G...3'
3'...G C ↑ T A G C...5' |
3’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15604S
|
LightNing® PvuII |
|
|
CAGCTG
5'...C A G ↓ C T G...3'
3'...G T C ↑ G A C...5' |
blunt |
37℃ |
No (column purification) |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: impaired.
|
|
EG24503S
|
LightNing® RsaI |
AfaI |
Csp6I, CviQI, RsaNI |
GTAC
5'...G T ↓ A C...3'
3'...C A ↑ T G...5' |
blunt |
37℃ |
No (column purification) |
Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15565S
|
LightNing® SacI |
Psp124BI, SstI |
Ecl136II, EcoICRI, Eco53kI |
GAGCTC
5'...G A G C T ↓ C...3'
3'...C ↑ T C G A G...5' |
3’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15605S
|
LightNing® SacII |
Cfr42I，KspI，Sfr303I，SgrBI |
|
CCGCGG
5'...C C G C ↓ G G...3'
3'...G G ↑ C G C C...5' |
3’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15566S
|
LightNing® SalI |
|
|
GTCGAC
5'...G ↓ T C G A C...3'
3'...C A G C T ↑ G...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: some blocked.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15568S
|
LightNing® SbfI |
SdaI, Sse8387I |
|
CCTGCAGG
5'...C C T G C A ↓ G G...3'
3'...G G ↑ A C G T C C...5' |
3’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG24502S
|
LightNing® ScaI |
ZrmI, BmcAI |
|
AGTACT
5'...A G T ↓ A C T...3'
3'...T C A ↑ T G A...5' |
blunt |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: blocked.
|
|
EG24515S
|
LightNing® SdaI (SbfI) |
SbfI, Sse8387I |
|
CCTGCAGG
5'...C C T G C A ↓ G G...3'
3'...G G ↑ A C G T C C...5' |
3’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15570S
|
LightNing® SfiI |
|
|
GGCCNNNNNGGCC
5'...G G C C N N N N ↓ N G G C C...3'
3'...C C G G N ↑ N N N N C C G G...5' |
3’ protruding ends |
50℃ |
No (column purification) |
Dam: no effect.
Dcm: impaired.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15572S
|
LightNing® SmaI |
|
Cfr9I, TspMI, XmaI |
CCCGGG
5'...C C C ↓ G G G...3'
3'...G G G ↑ C C C...5' |
blunt |
25℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG24521S
|
LightNing® SnaBI |
Eco105I, BstSNI |
|
TACGTA
5'...T A C ↓ G T A...3'
3'...A T G ↑ C A T...5' |
blunt |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15574S
|
LightNing® SpeI |
AhlI，BcuI |
|
ACTAGT
5'...A ↓ C T A G T...3'
3'...T G A T C ↑ A...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: blocked.
EcoBI: blocked.
|
|
EG15575S
|
LightNing® SphI |
PaeI |
|
GCATGC
5'...G C A T G ↓ C...3'
3'...C ↑ G T A C G...5' |
3’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: blocked.
|
|
EG15576S
|
LightNing® SspI |
|
|
AATATT
5'...A A T ↓ A T T...3'
3'...T T A ↑ T A A...5' |
blunt |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15577S
|
LightNing® StuI |
Eco147I, PceI, SseBI |
|
AGGCCT
5'...A G G ↓ C C T...3'
3'...T C C ↑ G G A...5' |
blunt |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: blocked.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15580S
|
LightNing® TaqI |
|
|
TCGA
5'...T ↓ C G A...3'
3'...A G C ↑ T...5' |
5’ protruding ends |
65℃ |
No (column purification) |
Dam: blocked.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG24517S
|
LightNing® Tsp45I |
NmuCI, TseFI |
|
GTSAC
5'...↓G T S A C ...3'
3' ... C A S T G↑...5' |
5’ protruding ends |
65℃ |
No (column purification) |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: blocked.
|
|
EG15581S
|
LightNing® XbaI |
|
|
TCTAGA
5'...T ↓ C T A G A...3'
3'...A G A T C ↑ T...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: blocked.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15582S
|
LightNing® XcmI |
|
|
CCANNNNNNNNNTGG
5'...C C A N N N N N ↓ N N N N T G G...3'
3'...G G T N N N N ↑ N N N N N A C C...5' |
3’ protruding ends |
37℃ |
80℃，20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG15583S
|
LightNing® XhoI |
PaeR7I, Sfr274I, SlaI |
|
CTCGAG
5'...C ↓ T C G A G...3'
3'...G A G C T ↑ C...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG24506S
|
BsiWI |
PspLI, Pfl23II |
|
CGTACG
5'...C ↓ G T A C G...3'
3'...G C A T G ↑ C...5' |
5’ protruding ends |
55℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG22507V
|
BsmBI |
Esp3I |
|
CGTCTC(1/5)
5'...C G T C T C (N)?↓...3'
3'...G C A G A G (N)?↑...5' |
5’ protruding ends |
55℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: some blocked.
EcoKI: no effect.
EcoBI: impaired.
|
|
EG23503S
|
BspQI |
SapI, LguI, PciSI |
|
GCTCTTC(1/4)
5'...G C T C T T C (N)?↓...3'
3'...C G A G A A G (N)?↑...5' |
5’ protruding ends |
50℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG25506S
|
BsrDI |
Bse3DI, BseMI |
|
GCAATGNN
5'...G C A A T G N N ↓...3'
3'...C G T T A C↑N N ...5' |
3’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: some blocked.
|
|
EG24516S
|
BstXI |
|
|
CCANNNNNNTGG
5'...C C A N N N N N ↓ N T G G...3'
3'...G G T N ↑ N N N N N A C C...5' |
3’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: impaired.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG25514S
|
PciI |
PscI |
|
ACATGT
5'...A ↓ C A T G T...3'
3'...T G T A C ↑ A...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: some blocked.
EcoBI: some blocked.
|
|
EG21501S
|
Sgel |
|
|
??CNNG(9/13)
5'...?? C N N G(N)? ↓...3'
3'... G N N C(N)?? ↑...5'*
*SgeI 可識別與切割含有5-mC位點的DNA靶標序列，一條鏈或雙鏈甲基化均可被識別。 |
3’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG25513S
|
SgrAI |
|
|
CRCCGGYG
5'...C R ↓ C C G G Y G...3'
3'...G Y G G C C ↑ R C...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: some blocked.
EcoBI: some blocked.
|
|
EG24514S
|
SspDI (KasI) |
KasI |
DinI, EgeI, EheI, Mly113I, NarI, PluTI, SfoI |
GGCGCC
5'...G ↓ G C G C C...3'
3'...C C G C G ↑ G...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG21504S
|
Esp3I(BsmBI), ADCF |
BsmBI |
|
CGTCTC(1/5)
5'...C G T C T C (N)?↓...3'
3'...G C A G A G (N)?↑...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG22504S
|
EcoRI, ADCF |
FunII |
|
GAATTC
5'...G ↓ A A T T C...3'
3'...C T T A A ↑ G...5' |
5’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: impaired.
|
|
EG23514S
|
Nb.BsrDI |
|
|
GCAATGNN
5'...G C A A T G N N...3'
3'...C G T T A C↑N N ...5' |
|
65℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG23512S
|
Nt.BspQI |
|
|
GCTCTTCN
5'...G C T C T T C N↓...3'
3'...C G A G A A G N ...5' |
|
50℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG24504S
|
Nt.BstNBI |
|
|
GAGTCNNNNN
5'...G A G T C N N N N↓N...3'
3'...C T C A G N N N N N ...5' |
|
55℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
|
EG24505S
|
MazF |
|
|
ACA
5'...↓ A C A ...3' |
5’ protruding ends |
37℃ |
|
|
|
EG25504S
|
I-SceI |
|
|
TAGGGATAACAGGGTAAT
5'...T A G G G A T A A ↓ C A G G G T A A T...3'
3'...A T C C C ↑ T A T T G T C C C A T T A...5' |
3’ protruding ends |
37℃ |
80℃, 20 min |
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
|
